What scientific breakthrough, related to germ theory, was developed about fifty years after Appert's success?
Answer
The work of Louis Pasteur
Appert’s method was a triumph of observation, as he applied heat to destroy spoilage agents without understanding the microscopic cause of decay. The scientific explanation for why his thermal process worked—the destruction of living microorganisms—was not established until much later. This explanation came through the meticulous research of Louis Pasteur, who articulated the germ theory, a concept that lagged approximately fifty years behind Appert's practical implementation of heat treatment.

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