What scientific breakthrough, related to germ theory, was developed about fifty years after Appert's success?

Answer

The work of Louis Pasteur

Appert’s method was a triumph of observation, as he applied heat to destroy spoilage agents without understanding the microscopic cause of decay. The scientific explanation for why his thermal process worked—the destruction of living microorganisms—was not established until much later. This explanation came through the meticulous research of Louis Pasteur, who articulated the germ theory, a concept that lagged approximately fifty years behind Appert's practical implementation of heat treatment.

What scientific breakthrough, related to germ theory, was developed about fifty years after Appert's success?

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Nicolas Appert and the Invention of Canning - YouTube

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