Which enzyme must act on Glucoraphanin to produce biologically active Sulforaphane (SFN)?

Answer

Myrosinase

The transformation of Glucosinolates (GSLs), specifically glucoraphanin, into biologically active compounds requires enzymatic action. Myrosinase is the enzyme responsible for this critical conversion. When broccoli tissue is damaged, such as through chewing or chopping, myrosinase becomes active and catalyzes the conversion of glucoraphanin into Sulforaphane (SFN). This activation step is essential because SFN, a potent antioxidant and anti-inflammatory agent, is not readily available until this chemical reaction takes place. The enzyme's activity is the rate-limiting step for maximizing SFN bioavailability from the vegetable.

Which enzyme must act on Glucoraphanin to produce biologically active Sulforaphane (SFN)?
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