Which chemical methods quantify nitrogen content as a proxy to determine protein content?
Answer
Kjeldahl or Dumas methods
In the realm of direct chemical determination, quantifying protein content in a food matrix requires specialized techniques because direct measurement of protein itself can be complex. The established standards for this quantification involve methods that measure nitrogen, which is present in all proteins, and then use a known conversion factor to estimate the total protein. Specifically, the Kjeldahl method and the Dumas method are the referenced techniques utilized by researchers and labs to isolate and quantify this nitrogen content, thus providing a reliable proxy value for the actual protein percentage within the food sample.
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